alpha-Glucosyl Hesperidin

Product name

α-Glucosyl Hesperidin;

Alpha-Glucosyl Hesperidin;

Enzymatically Modified Hesperidin;

Hesperidin Glucoside;

CAS No.

161713-86-6

Molecular Formula

C34H44O20

Molecular Weight

772.711

Quality Standard

Assay, on dried substance

- α-Monoglucosyl hesperidin ≥ 75% by HPLC

Appearance

Pale yellow to yellowish brown powder

Test Items

Specifications

Results

Appearance

Pale yellow to yellowish brown powder

Pale yellow powder

Solubility

Freely soluble in water and methanol, slightly soluble in ethanol (96 per cent).

Conforms

Identification

Infrared absorption spectrophotometry

Comparison: α-Glucosyl Hesperidin RS .

Conforms

UV/Vis absorption spectrophotometry

UV spectrum of test solution in a range from 240 nm to 500 nm must have a maximum at a wavelength of280 nm ~ 286 nm.

Conforms

Examine the chromatograms obtained in the Assay by HPLC

The principal peak in the chromatogram

obtained with the test solution is similar in retention time to the principal peak in the chromatogram obtained with reference solution.

Conforms

Appearance and colour of solution, 1.0g/10mL water

Light yellow to yellow clear solution

Light yellow clear solution

Loss on drying

≤ 5.0%

2.3%

Sulphated Ash

≤ 0.50%

0.10%

Heavy metals, as Pb

≤ 20ppm

< 20ppm

Heavy metals

Arsenic(As)

≤ 1.5ppm

< 0.01ppm

Lead(Pb)

≤ 0.5ppm

< 0.01ppm

Cadmium (Cd)

≤ 0.5ppm

< 0.05ppm

Mercury(Hg)

≤ 3.0ppm

< 0.01ppm

ASSAY, 

on dried substance

- α-Monoglucosyl hesperidin, by HPLC

≥ 75%

82.7%

Particle size

≥ 97% of the powder by mass passes through a number 180 sieve (80 mesh sieve)

> 98%

Density of powders

Bulk density

0.40 g/mL ~ 0.60 g/mL

0.54 g/mL

Tapped density

0.65 g/mL ~ 0.85 g/mL

0.77 g/mL

Residual solvents 

Methanol

≤ 3000 ppm

21ppm

Ethanol

≤ 5000 ppm

23ppm

Microbiological

Total aerobic microbial count

≤1000cfu/g

< 100cfu/g

Total yeast&mold

≤100cfu/g

< 10cfu/g

Escherichia Coli

Absent in 1 g

Absent in 1 g

Conclusion

The product complies with the specification