Trometamol

Details:

1. Introduction of Trometamol

Trometamol is an abbreviation of the organic compound known as tris(hydroxymethyl)aminomethane, with the formula (HOCH2)3CNH2. Tris is extensively used in biochemistry and molecular biology.In biochemistry, Tris is widely used as a component of buffer solutions, such as in TAE and TBE buffer, especially for solutions of nucleic acids. It is a primary amine and thus undergoes the reactions associated with typical amines, e.g. condensations with aldehydes.

2. Properties of Trometamol

ACD/LogP: -2.515 # of Rule of 5 Violations: 1
ACD/LogD (pH 5.5): -5.57 ACD/LogD (pH 7.4): -4.67
ACD/BCF (pH 5.5): 1.00 ACD/BCF (pH 7.4): 1.00
ACD/KOC (pH 5.5): 1.00 ACD/KOC (pH 7.4): 1.00
#H bond acceptors: 4 #H bond donors: 5
#Freely Rotating Bonds: 7 Polar Surface Area: 86.71  2
Index of Refraction: 1.544 Molar Refractivity: 28.686 cm3
Molar Volume: 90.806 cm3 Polarizability: 11.372 10-24cm3
Surface Tension: 73.2040023803711 dyne/cm Density: 1.334 g/cm3
Flash Point: 169.706 °C Enthalpy of Vaporization: 69.73 kJ/mol
Boiling Point: 356.998 °C at 760 mmHg

3. Structure descriptors of Trometamol

IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol

InChI: InChI=1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChIKey: LENZDBCJOHFCAS-UHFFFAOYSA-N

Canonical SMILES : C(C(CO)(CO)N)O

4. Uses of Trometamol

The useful buffer range for tris (7-9) coincides with the physiological pH typical of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory. Tris is also used as a primary standard to standardize acid solutions for chemical analysis.

5. Medical

Trometamol(usually known as THAM in this context) is used as alternative to sodium bicarbonate in the treatment of metabolic acidosis.

Tris is one of the most widely used buffers in molecular biology and cell culture due to its low toxicity, stability and buffering capacity