PIPES;1,4-Piperazinediethanesulfonic acid;

Details:

PIPES [piperazine-N,N′-bis(2-ethanesulfonic acid)] is frequently used as a buffering agent in biochemistry. It is an ethanesulfonic acid buffer developed by Good et al. in the 1960s. PIPES has a pK_a near the physiological pH which makes it useful in cell culture work. It has been documented to minimize lipid loss when buffering glutaraldehyde histology in plant and animal tissues. 

Additional forms available: PIPES, Sesquisodium Salt; PIPES dipotassium salt; PIPES disodium salt; PIPES, Sodium Salt . Buffers can be prepared by adding a solution of base to PIPES free acid, titrating to the appropriate pH, or by mixing equimolar solutions of the monosodium salt and the disodium salt, titrating to the appropriate pH.

Product ID: TQBB026

Name: PIPES

Specification/price: 25KG / DRUM

Rank: Ultra Pure Grade

CAS: 5625-37-6

English Name: Piperazine-1,4-bisethanesulfonic acid

Molecular Formula:C8H18N2O6S2

Molecular Wt:302.4

Appearance: Free flowing white powder

Assay(potertiometric cal on dry substance): 99.5％

Enterprise Standard

Application: it is an important PH stable reagent in biotic experiments.

Appropriate PH is obtained by mixing mild acid and its conjugate base.

Many biological responses happen in neutral condition (usually PH 6-8),

so the buffering range is required to be in PH 6-8. In addition, the form

of acid-base of buffer should not chelate with some metallic ions.

Characteristics of biological buffer:

1. Strong water-solubility;

2. Compatibility of the acid-base dissociation balance;

3. Low membrance penetration rate;

4. Low metal chelating ability;

5. High chemical stability;

6. Low absorption in ultraviolet and visible region.