Add time:07/19/2019         Source:sciencedirect.com

We tested 1-methyl-2-nitrosoimidazole (IN% the two electron reduction product of 1-methyl-2-nitroimidazole (INO2) for its in vitro cytotoxicity and glutathione (GSH) depleting capabilities. The half life of INO was shown to be dependent on cell concentration above 105 cells/ml, decreasing with increasing cell concentration up to 2 × 106 cells/ml. For a 10-fold decrease in cell concentration, from 106 to 105 cells/ml, the toxicity curve shifted 10-fold towards lower concentrations. At 106 cells/ml, INO depleted GSH, in the range of concentrations where toxicity was observed, down to a plateau of 15% of the control level at a concentration of 100 μM INO. Oxidized glutathione (GSSG) levels were not elevated significantly above control cultures at this concentration. INO2, 1000 μM, did not deplete GSH under similar exposure conditions while 2-hydroxylamino-l-methylimidazole (INHOH) depleted GSH minimally at this same concentration. The nitroso intermediate may play a central role in the toxicity and GSH depleting capabilities of 2-nitroimidazoles in mammalian cells.

We also recommend Trading Suppliers and Manufacturers of 1-methyl-2-nitrosoimidazole (cas 116169-87-0). Pls Click Website Link as below: cas 116169-87-0 suppliers