Add time:07/21/2019         Source:sciencedirect.com

Guinea-pig vasa deferentia were incubated with a 2-halogenoalkylamine (14C-SY28) either alone or after exposure to the alpha receptor blocking agent, phentolamine. A quantitative evaluation was made of the distribution of the covalently bound 14C-SY28 both in lipid and lipid-free phases, and the distribution throughout the subcellular fractions of lipid-free homogenate was studied. Acid hydrolysis was carried out on each fraction and hydrolysates run on chromatography paper. Autoradiographic studies of these runs showed 14C-SY28-amino acid complexes, identical to those found previously, distributed unequally throughout the subcellular fractions. The effects of phentolamine protection of the alpha receptor, from binding by 214C-SY28 are discussed.

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