Add time:07/29/2019 Source:sciencedirect.com
A co-metabolic degradation of Tribenuron (cas 106040-48-6) methyl bacterial strain NyZ42 was isolated from polluted agricultural soil and classified as genus Pseudomonas by its 16S rRNA gene sequencing. The degradation efficiency of tribenuron methyl was about 80% of the originally supplemented 200 mg l−1 tribenuron methyl in liquid minimal medium within four days, when either glucose or succinate was used as a supplemental carbon source. Three intermediates formed during the degradation of tribenuron methyl mediated by strain NyZ42 were captured by LC-MS, and two alternative pathways were proposed for the microbial mediated tribenuron methyl degradation, via either cleavage of the sulfonylurea bridge or saponification of alkyl-group. Furthermore, inoculation of strain NyZ42 enhanced the degradation of tribenuron methyl in the sterilized soil samples, although the biodegradation/co-metabolism ability of NyZ42 was not obvious in the nonsterilized soil samples when compared with the indigenous microbial consortium under current laboratory conditions.
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