Add time:07/23/2019         Source:sciencedirect.com

An enzyme incubation–water extraction (EI–WE) method was developed and optimised for the extraction of the natural antioxidant taxifolin and of the total flavonoids from wood sawdust of Larix gmelini (Rupr.) Rupr. A factorial design and a central composite design approach were used for method optimisation. Optimal conditions were 0.5 mg/ml cellulase and 0.5 mg/ml pectinase, a pH of 5.0, a temperature of 32 °C and 18 h incubation time. The flavonoids and taxifolin were extracted in hot water at 50 °C for 30 min, with a solid to liquid ratio of 1:20. Under optimised conditions, the yields of taxifolin and total flavonoids increased from 1.06 ± 0.08 to 1.35 ± 0.04 mg/g and 4.13 ± 0.17 to 4.96 ± 0.29 mg/g, respectively. DPPH and BHT assays revealed that the EI–WE samples had 1.8- and 1.68-fold higher antioxidant activities than the controls. SEM results revealed the structural disruption of wood sawdust with enzyme incubation.

► Organic solvent free. ► Combined enzyme system was used for the extraction of taxifolin and total flavonoids in Larix gmelini (Rupr.) Rupr. sawdust. ► Taxifolin extracted by EI–WE method can be used as food additive for its excellent antioxidant activity. ► CCD (central composite design) was used for the optimisation of taxifolin and total flavonoids extraction condition. ► Realise material recycle, for L. gmelini (Rupr.) Rupr. sawdust is generally deserted as castoff.

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