Add time:07/25/2019         Source:sciencedirect.com

Choline uptake and incorporation into Schistosoma mansoni is used as a model for investigating transport across and formation of a double bilayer surface of a syncytial transporting epithelium. Choline uptake reached a maximal rate during the first 2 min (Vmax = 0.27 μmol mg−1 protein min−1; Km = 36 μM). Choline uptake during a 30 min incubation was similar to that of single bilayer transport systems described in the literature. Choline incorporation into phosphatidylcholine was saturated above 40 μM external choline concentration (Vmax = 3.7 pmol mg−1proteinmin−1; Km = 7 μM). The low rate of choline efflux and the half life of the tissue choline pool (T12 = 3 h), suggests that free choline pools available for efflux in S. mansoni are small. This model allows the determination of whether a proposed effector of membrane phosphatidylcholine synthesis and turnover alters surface bilayer formation through changes in transport of the precursor across the apical surface.

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