Add time:07/29/2019         Source:sciencedirect.com

The specific radioactivity of a choline phospholipid has been determined by a double-isotope method. Purified phospholipid was hydrolyzed to release labeled choline, and choline kinase was employed to label the choline with 32P from [γ-32P]ATP. The double-labeled phosphorylcholine was purified by ion-exchange chromatography on QAE-Sephadex, and the specific radioactivity of the choline was calculated from the isotope ratio. The method is sensitive, requiring only 5 nmol of choline with a specific radioactivity of 1 μCl/μmol, and the chromatographic isolation of phosphorylcholine is simple and reproducible.

We also recommend Trading Suppliers and Manufacturers of CHOLINE CHLORIDE, [1,2-14C] (cas 109572-96-5). Pls Click Website Link as below: cas 109572-96-5 suppliers