Add time:07/25/2019         Source:sciencedirect.com

A novel nanocomposite of silver/titanium dioxide/chitosan adipate (Ag/TiO2/CS) was developed through photochemical reduction using a chitosan adipate template. Chitosan served as a reducing agent anchoring the metal ions through Ag–N coordination bonds and electrostatic attractions, thus stabilizing the Ag/TiO2/CS product observed by Fourier transform infrared spectroscopy and X-ray diffraction patterns. Scanning electron microscopy observations revealed that the nanocomposite particles (50–100 nm) were deposited onto the chitosan adipate layer. The product exhibited high ζ-potentials from 30.1 mV to 33.0 mV during 60 days of storage. In addition, the nanocomposite demonstrated higher antimicrobial activity than AgNO3 or nano-Ag particles at similar concentrations, as evidenced by the inhibition zone, minimum inhibitory concentrations (MIC), and growth curve. The nanocomposite reduced the Escherichia coli population by 6 logs after 24 h of incubation, and had an MIC value of 0.38 μg Ag/mL. These results suggest that Ag/TiO2/CS has the potential to be used as an antibacterial protective coating for fruit storage.

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