Add time:07/24/2019         Source:sciencedirect.com

A glycosyltransferase gene, rhoG, involved in the biosynthesis of the anthracycline antibiotic β-rhodomycin was isolated as a 4.1-kb DNA fragment containing rhoG and its flanking region from Streptomyces violaceus by degenerate and inverse PCR. Sequencing analysis showed that rhoG was located in a gene cluster involved in the biosynthesis of the constitutive deoxysugar of β-rhodomycin. The function of rhoG was verified by gene disruption, which was generated by replacing the internal 0.9-kb region of S. violaceus chromosome with a fragment including the SacI-blunted region. The rhoG disruption resulted in complete loss of β-rhodomycin productivity, along with the accumulation of a non-glycosyl intermediate ϵ-rhodomycinone. In addition, the complementation test demonstrated that rhoG restored β-rhodomycin production in this gene disruptant. These results indicated that rhoG is the glycosyltransferase gene responsible for the glycosylation of ϵ-rhodomycinone in β-rhodomycin biosynthesis.

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