Add time:07/24/2019         Source:sciencedirect.com

An efficient four step synthesis of the title compound 4-hydroxy-3-oxobutylphosphonate (2) has been developed based on inexpensive 4-ethoxy-1-hydroxybutane-2-one using an Arbusow reaction (59% overall yield). Several dihydroxyacetone-dependent aldolases having different stereospecificities were tested for their acceptance of this phosphonomethyl substrate mimic as the aldol donor. Individual enzymes belonging to both type I (Schiff base formation) and type II (Zn2+ catalysis) mechanistic classes were found to catalyze the stereoselective addition of 2 to simple aldehydes to provide bio-isosteric analogs of sugar 1-phosphates in high yields. The lack of acceptance by specific enzymes is discussed with regard to recent protein X-ray data.

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