Add time:07/27/2019 Source:sciencedirect.com
Resonance Raman spectra of 2-(4′-hydroxyphenylazo)-benzoic acid have been recorded for aqueous and ethanolic solutions. The change of pH of the aqueous solution gives four types of spectra corresponding to the stages of protonation and deprotonation processes. On addition of bovine serum albumin or cetyltrimethylammonium bromide to the neutral solution, the spectrum shows a little change which can be elucidated based on the shift of equilibrium between the azo- and the hydrazone-forms. The spectrum of the ethanolic solution shows a marked concentration dependence.
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