Add time:08/02/2019 Source:sciencedirect.com
The recently developed method of loading isolated heart mitochondria with the fluorescent pH indicator, BCECF, was applied to monitor the Na+0/H+i exchange process from the matrix side of the membrane. The Na+-induced changes in the pH of the matrix (pHm) showed that: (i) the Na+0/H+i exchange followed Michaelis-Menten kinetics with respect to external Na+ with a Km of approx. 20 mM; (ii) in contrast to this, the dependence of the exchange rate on the matrix [H+] did not obey the Michaelian model. No Na+-induced alkalinization occurred above a pHm of 7.45±0.09 (n=4). Below this value the reciprocal of the transport rate and that of the matrix [H+] deviated upwardly from the straight line. The results suggest that internal H+ might exert allosteric control on the mitochondrial Na+/H+ exchange process.
We also recommend Trading Suppliers and Manufacturers of BCECF-AM (cas 134331-92-3). Pls Click Website Link as below: cas 134331-92-3 suppliers
About|Contact|Cas|Product Name|Molecular|Country|Encyclopedia
Message|New Cas|MSDS|Service|Advertisement|CAS DataBase|Article Data|Manufacturers | Chemical Catalog
©2008 LookChem.com,License: ICP
NO.:Zhejiang16009103
complaints:service@lookchem.com Desktop View