Add time:07/26/2019         Source:sciencedirect.com

Several derivatives of 1-methyl-4-phenylpyridinium (MPP+), i.e., 1-methyl-4-(4′-nitrophenyl)pyridinium (1), 1-methyl-4-(4′-cyanophenyl)pyridinium (2), 1-methyl-4-(3′-nitrophenyl)pyridinium (3), 1-methyl-4-(4′-chlorophenyl)pyridinium (4), 1-methyl-4-(4′-acetamidophenyl)pyridinium (5), and 1-methyl-4-(4′-aminophenyl)pyridinium (6), were synthesized in order to compare their toxicity with that of paraquat (PQ2+) in Escherichia coli. Addition of compounds 1, 2, and 3 to aerobic E. coli cell suspensions caused extracellular ferricytochrome c reduction, which was inhibited by superoxide dismutase in the same manner as that in the case of PQ2+. The rate of the ferricytochrome c (cyt. c) reduction was in the order of PQ2+ > 1 > 2 > 3, which is the same as that of the redox potentials of these compounds. On the other hand, MPP+, 4, 5, and 6, which have more negative potentials, had no effect on the cyt. c reduction. Compound 1 inhibited the growth of E. coli under aerobic conditions, but not under anaerobic conditions. The results show that compound 1 can act as a mediator for production of Superoxide (O⨪2), which seriously injures E. coli cells. However, though compounds 2 and 3 catalyzed the production of ⨪2 in E. coli cells, their activity of ⨪2 production was much lower than that of compound 1 or PQ2+. Thus, compound 3 had no effect on growth or survival of E. coli at 1 mm, while compounds 2 and 4 had both bacteriostatic and bacteriocidal effects which were independent of dioxygen (o2). The results show that the toxic mechanism is different from that of compound 1. MPP+, 5, and 6 had no effect on growth of E. coli. This paper shows that compound 1 is a novel enhancer of intracellular Superoxide production, though the mechanism of toxicity of compounds 2 and 4 is not clear yet. The results suggest that the redox potential is a crucial factor for manifestation of the activity.

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