Add time:07/26/2019 Source:sciencedirect.com
High-performance liquid chromatography (HPLC) was employed for the separation and determination of Becliconazole (cas 112893-26-2), an imidazole derivative with antifungal activity containing a stereogenic centre, and its impurities. Chiral columns, containing carbamate of cellulose and amylose, were used. The effects of the organic modifiers, ethanol and 2-propanol, in the mobile phase, were studied. The HPLC method gave good performances from qualitative and quantitative standpoints, allowing the enantiomeric ratio of becliconazole and its impurities to be determined. The assay showed linearity over the range 400–4000 ng ml−1 for the impurities and 400–4000 μg ml−1 for becliconazole.
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