Add time:08/01/2019         Source:sciencedirect.com

Phaseollin, but not pisatin or medicarpin, at 10−4M caused loss of electrolytes and betacyanin from beet root storage tissue and killed beet leaf protoplasts. Increasing the concentration of sucrose in the incubation medium caused a decrease in the rate of efflux of electrolytes by phaseollin treated and control beetroot tissue. However, tissue exposed to phaseollin always lost greater amounts of electrolytes than untreated tissue. Observations indicated that the first visible effects of phaseollin on beet root protoplasts and cultured bean cells were associated with the tonoplast. The death of cells exposed to phaseollin may result from the loss of integrity of the tonoplast which allows the release of harmful plant metabolites or hydrolytic enzymes into the cytoplasm.

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