Add time:07/30/2019         Source:sciencedirect.com

The association of PONASTERONE A (cas 13408-56-5) (PNA) and 20-hydroxyecdysone with Kc cells is commensurate with their biological activity on this Kc cell line, the physiological activity ratio for PNA, 20-hydroxyecdysone and ecdysone is 1:50:2000, resp. Both association and release of [3H]-PNA are temperature-dependent, the activation energy was calculated as 16.7 cal (Arrhenius analysis). This association is compatible with unlabelled PNA and various ecdysteroids. The KD for PNA (Scatchard analysis) was estimated as 3.6 × 10−9 M, giving the number of binding sites as approx. 1800 per cell.

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