Add time:07/26/2019         Source:sciencedirect.com

Adenyl cyclase activity was measured in washed ejaculated spermatozoa of the rhesus monkey (Macaca mulatta) by monitoring the conversion of [14C]ATP to [14C]cyclic 3′,5′-AMP. Thyroxine and triiodothyronine increase the accumulation of cyclic 3′,5′-AMP; other hormones, including androgens, gonadotropins, prostaglandins, and catecholamines had no effect. Manganese ion, over the range 0.5–20 mm, stimulated adenyl cyclase activity 2- to 50-fold, but in parallel studies inhibited fructolysis and motility. Testosterone, dihydrotestosterone, and epinephrine stimulated the accumulation of cyclic 3′,5′-AMP 60–70% after preincubation of the cells with triiodothyronine while androstenedione and progesterone had no effect under the same conditions.Spermatozoal intracellular cyclic 3′,5′-AMP, measured using cyclic 3′,5′-AMP-dependant protein kinase, averaged 103 ± 28 pmoles/109 spermatozoa. Intracellular cyclic 3′,5′-AMP content was not altered by treatment with single hormones but combinations of triiodothyronine and dihydrotestosterone, in some instances, increased cyclic 3′,5′-AMP levels almost 2-fold. Manganese ion, at concentrations which stimulate adenyl cyclase activity, decreased the intracellular content of cyclic 3′,5′-AMP by 40% after incubation for 1 hr at 37 °.

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