Add time:07/26/2019         Source:sciencedirect.com

The measurement of the fluorescence emitted by flavones and flavonols in situ on thin-layer chromatography plates can be improved by dipping the developed plates in appropriate solutions. The effect of different substances on the intensity and stability of the fluorescence of 14 flavone and 26 flavonol derivatives detected with diphenylboric acid 2-aminoethyl ester (Naturstoffreagens A, NA) is described. Silicone oil or paraffin is most appropriate for lipophilic flavonoids while the more hydrophilic polyethylene glycol 4000 is most appropriate for less lipophilic aglycones as well as glycosides. Authentic reference standards in appropriate concentrations must be measured on the same plate as the experimental sample since flavonoids demonstrate specific fluorescence behaviour and because in situ fluorodensitometry measurements are difficult to reproduce. The correlation between fluorescence and molecular structure is discussed briefly. The test conditions established are an improvement on the accepted in situ fluorodensitometry determination of flavonoids.

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