Add time:07/28/2019         Source:sciencedirect.com

1.1. Rat-thymus cells were incubate with [14C6]glucose. Ribose and deoxyribose moieties bound to the different bases of nucleic acids were isolated and the incorporation of 14C into these sugars was determined.2.2. Among the ribose moieties, those bound to adenine and guanine had the highest specific activities; that of uracil-bound ribose was lower, while the specific activity of the cytosine-bound ribose was the lowest, amounting to only a few per cent of that of the purine-ribose.3.3. In deoxyribonucleic acid, similarly, the specific activity of the purine-deoxyribose was much higher (about 20 times) than that of the pyrimidine-deoxyribose.4.4. On the other hand, when 32P was used as a precursor, no such differences were observed in its uptake into the 5′-nucleotides derived from ribonucleic acid, nor in its uptake into the deoxyribonucleic acid nucleotides.5.5. These results are discussed in relation to the comparative utilization of the purine and pyrimidine nucleosides as precursors in the formation of nucleic acids.

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