Add time:07/27/2019 Source:sciencedirect.com
A method for determining a selective muscarinic agent, LY297802 (compound I), [(3-(3-1-butylthio)-1,2,5-thiadiazol-4-yl)-1-azabicyclo[2.2.2]octane], indicated in the treatment of pain, in rat, rabbit, and monkey plasma is described. The analytes, including an internal standard, were extracted from plasma at basic pH with hexane. The organic fraction was evaporated to dryness and the residue reconstituted with mobile phase. The analytes were detected utilizing HPLC in conjunction with electrospray (ES) tandem mass spectrometry (MS-MS). The limit of quantitation was 0.25 ng/ml, and the response was linear to at least 100 ng/ml.
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