Add time:07/28/2019         Source:sciencedirect.com

Biosynthesis of melanin byColletotrichum lagenariumis essential for appressorial penetration of its host plants. Carpropamid is a novel melanin-inhibiting fungicide for control of rice blast caused byMagnaporthe grisea.InC. lagenarium,appressorial and colonial melanization were inhibited by carpropamid. Accumulation of melanin intermediates, scytalone, was also shown in the culture medium containing carpropamid. One of the enzymes involved in the melanin biosynthesis, scytalone dehydratase catalyzes the conversion of scytalone to 1,3,8-trihydroxynaphthalene. We previously clonedSCD1gene coding for scytalone dehydratase ofC. lagenarium.In this study, a recombinantSCD1expression vector was constructed using pMAL system and the recombinant scytalone dehydratase was purified from transformedEscherichia coli.Recombinant SCD1 showed scytalone dehydratase activity in anin vitroreaction using scytalone as substrate. The activity was assayed by measuring the production of 1,3,8-trihydroxynaphthalene or disappearance of scytalone, which was detectable by reverse-phase high-pressure liquid chromatography. Dehydration of scytalone to 1,3,8-trihydroxynaphthalene was inhibited by carpropamid in thein vitroreaction.

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