Add time:07/28/2019 Source:sciencedirect.com
Hydroxamic acids (HA), with the general formula R–CO–NHOH, are chelating agents which may be used in a number of interesting applications, such as medicine and waste water treatment. In this paper, we describe the enzymatic synthesis of HA with various chain lengths (from C2 to C18), using three microbial enzymes. For short- and middle-chain HA synthesis, using amidases from Rhodococcus sp. R312, the optimal working pH was found to be pH 7 or 8, depending on the amide substrate used. Different Michaelis–Menten constants were also determined. For fatty HA synthesis, using lipase from Candida parapsilosis, the optimal working conditions were determined to be pH 6, 1 M hydroxylamine and 40°C. Because of the favorable bioconversion yields achieved, the enzymatic synthesis of HA with the appropriate biocatalysts appears to be an interesting alternative to chemical synthesis.
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