Add time:07/29/2019         Source:sciencedirect.com

The electrode sensitive to dibenzyl dimethyl ammonium (DDA+), which is considered to be an indicator of the membrane potential, was constructed by using tetraphenyl borone (TPB−) embedded in dichloroethane. Rapid and Nernstian responses were exhibited against DDA+ solutions ranging between 10−2 and 3 · 10−6 M in concentration. High selectivity for DDA+ was observed in the presence of various inorganic salts, ADP, ATP, oxidizable substrates and sugars. The electrode developed here was used to measure the DDA+ uptake in Streptococcus faecalis and the results agreed with those reported by Harold, F.M. and Papineau, D. ((1972) J. Membrane Biol. 8, 27–44 and 45–62). While they determined the DDA+ concentration in the medium by measuring the absorbance of the filtrate treated with the ion-exchangers, the electrode can measure directly the DDA+ concentration in the bacterial suspension without any pretreatment. It was also shown that the electrode can measure the DDA+ uptake in mitochondria during energization.

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