Add time:08/02/2019         Source:sciencedirect.com

SummaryUsing excised buckwheat cotyledons treated with glyphosate to block endogenous supply of aromatic amino acids a comparison of the precursor role of exogenous L-phenylalanine and L-tyrosine in building flavonoids was carried out. Glyphosate reduced biosynthesis of flavonoids from both endogenous and exogenous substrates. However, with increasing concentrations of the herbicide the relative incorporation of exogenous L-phenylalanine into flavonoids showed a clear-cut increase notwithstanding that in absolute units their biosynthesis from that precursor declined. No similar relative rise of incorporation was observed in feeding exogenous L-tyrosine. A conclusion is drawn that Ltyrosine is not an alternative natural precursor of flavonoids in buckwheat seedlings. Possible mechanisms responsible for the labelling of flavonoids from the [14C]-L-tyrosine supplied exogenously are discussed.

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