Add time:07/29/2019         Source:sciencedirect.com

Because of the localization of creatine in muscle, the feasibility of using isotopic dilution of 14C-creatine to estimate muscle mass in vivo was investigated. Rats were sacrificed at daily intervals following the administration of 1–2 μc. of 1-14C-creatine I.V. Urine and stool were the major routes of 14C excretion. 14C calculated to be remaining in the body (“14C injected minus 14C excreted”) was 100 ± 2 per cent of that found by carcass analysis. By 144 hours, 94 per cent of body 14C was in carcass muscle, 4 per cent in skin and 2 per cent in gut, kidney and liver. Although not all 14C was in muscle, the discrepancy was accepted since only a 6–7 per cent overestimate of muscle mass could be introduced. The mean 14C concentration in four muscle pieces was considered to be representative of the concentration in the muscle compartment. Paired comparison revealed that there was no significant difference between muscle mass values determined by isotopic dilution (14C remaining in the body divided by 14C/Gm. fat free muscle) and the anatomic muscle mass (obtained by dissection) in 24 rats weighing 200–400 Gm. These results indicate that dilution of 14C-creatine can be used to measure muscle mass in vivo in rats and suggest that with appropriate modification this method may be applicable to man and other large animals.

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