Add time:07/29/2019         Source:sciencedirect.com

Rough lemon rootstock seedlings were held in 14-l. plastic tanks containing solutions of the pigments dihydrofusarubin (DHF), Isomarticin (cas 19196-46-4) and a mixture (T-79; 80% DHF and 20% isomarticin) of the two extracted from cultures of Fusarium solani. Both pigments, together or alone, caused initial leaf roll and wilt symptoms within 48–72 h after exposure. The T-79 mixture was effective at the lowest rate, 25 μgl−1, causing wilt, a reduction in growth, a depression of solution uptake and veinal chlorotic patterns in leaves. Chlorosis was ascribed only to DHF in later tests, and it caused this symptom at the lowest rate tested, 10μgl−1. At 50μgl−1, each pigment had similar effects, but DHF increased vessel plugging in stems more than isomarticin. Both pigments caused a loss of water and electrolytes from leaves, indicating that they affected membrane integrity. Isomarticin and fusarubin, the oxidation products of DHF, were visually detected in stems and leaves. Of nine rootstocks tested with DHF, rough lemon appeared to be the most sensitive.

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