Add time:07/29/2019         Source:sciencedirect.com

A polarized laser (488.0 nm) Raman spectroscopic measurement has been made on a single crystal of a disodium salt of adenosine triphosphoric acid (Na2ATP·3H2O) by the use of a Raman microscope. The crystal belongs to an orthorhombic system of the space group P212121, and has dimensions of 10, 100 and 100 μm along the crystallographic axes a, b and c, respectively. For each Raman band in the 300–1800 cm−1 range, the scattering intensity ratio I bb/I cc of the bb and cc polarization components has been determined. For a few bands, the relative intensities of the bc components were also estimated. To augment the data, the depolarization ratio of each Raman band of ATP has also been determined for its acidic (pH = 2.42) H2O and D2O solutions. From these experimental results, the shapes and orientations of the Raman scattering tensors which are considered to be localized in the adenine-H+ portion and in the phosphate portion of the molecule have been derived.

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