Add time:08/01/2019         Source:sciencedirect.com

Application of a method for the “in situ” generation of 9-ANTHRYLDIAZOMETHANE (cas 10401-59-9) (ADAM) to the derivatization of the carboxyl function in diarrhetic shellfish poisoning (DSP) toxins revealed the formation of artifact products. Using liquid chromatography–mass spectrometry, it was determined that these artifacts were due to base-catalyzed reactions between the solvent, ethyl acetate, and the hydroxyl groups of the analyte to produce O-acetylated ADAM derivatives. Using a new formulation, with tetrahydrofuran as solvent, it was possible to eliminate these artifact reactions. Various reaction parameters have also been re-optimized to ensure quantitative derivatizations. An assessment method was developed that was useful not only for optimizing reaction parameters, but also for evaluating the reagent potency before use on important samples. Finally, application of the method to the determination of DSP toxins in plankton and mussel tissue was demonstrated.

We also recommend Trading Suppliers and Manufacturers of 9-ANTHRYLDIAZOMETHANE (cas 10401-59-9). Pls Click Website Link as below: cas 10401-59-9 suppliers