Add time:08/03/2019         Source:sciencedirect.com

Publisher SummaryModifications and alternatives to the original HPLC-EC (high-performance liquid chromatography- electrochemical) methods have produced a burgeoning literature on oxidative DNA damage. At the same time, they have resulted in discordant results and disagreements about the most appropriate techniques. Because of the apparent importance of DNA damage, and the interest in 8-Oxo-2'-deoxyguanosine (oxo8dG) and oxo8Gua as biomarkers for this damage, it is important to identify the best available assay methods. This chapter reviews the HPLC-EC methods that find to be the most reliable, indicate the common pitfalls associated with these techniques, and uncertainties of interpretation. Methods for DNA isolation continue to improve, with minor modification of the chaotropic NaI technique producing the lowest oxo8dG values reported to date using HPLC-EC. Although the use of urinary oxo8Gua adducts to monitor DNA oxidation in vivo remains attractive, the results can be difficult to interpret.

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