Add time:08/01/2019         Source:sciencedirect.com

1.1. Analytical differential centrifugation of brown adipose tissue homogenates from cold-acclimated guinea pigs revealed a polydispersity of both mitochondria and peroxisomes, with at least two populations of each organelle. The estimated values were s̄H = 16 685 ±4220 S and s̄L = 4792±951 S (mitochondria), and s̄H = 3 364 ±1706 S and s̄L = 889±177 S (peroxisomes). Based on these s̄ values, an optimal procedure is described for the isolation of subcellular fractions enriched in mitochondria and peroxisomes, respectively.2.2. When the mitochondrial and peroxisomal fractions were subjected to isopycnic gradient centrifugation on a self-generating gradient of poly(vinylpyrrolidone)-coated colloidal silica particles (Percoll) in 0.25 M sucrose medium, buoyant densities of about 1.11 g/cm3 (main fraction of mitochondria) and 1.07 g/cm3 (main fraction of peroxisomes) were obtained. A value of 1.06 g/cm3 was found for the microsomal fraction.3.3. The main peroxisomal fraction, isolated by gradient centrifugation, did not reveal any significant oxidation of palmitoyl-CoA as measured by conventional polarographic technique, whereas a small rate of oxidation (about 2.7 ±0.2 nmol/minpermg peroxisomal protein) was observed when measured as NAD+ reduction. This rate contributes no more than 1% of the mitochondrial oxidation of this fatty acid and it is, therefore, concluded that peroxisomal oxidation of the predominant long-chain fatty acids found in this tissue does not make a quantitatively significant contribution to fatty acid oxidation.

We also recommend Trading Suppliers and Manufacturers of C.I. Acid Brown 314 (cas 12219-81-7). Pls Click Website Link as below: cas 12219-81-7 suppliers