Add time:08/06/2019         Source:sciencedirect.com

Reversed-phase chromatography (RFC) was used to resolve two variants of recombinant amino terminal methionyl residue (TV-Met) insulin-like growth factor-I (IGF-I) with the same amino acid constitution but different disulphide linkages. Following radioiodination, equilibration with plasma and size exclusion chromatography at neutral pH the major form on RPC (approximate abundance 60%) demonstrated > 80% binding to 150 kDa and 40–50 kDa IGF binding proteins. This peptide has the RPC elution characteristics and disulphide assignment (Cys6-Cys48, Cys18-Cys61, Cys47-Cys52) of authentic IGF-I (termed TV-Met IGF-I peak 2 peptide). By contrast, a second peptide (approximate abundance 25%) with mismatched disulphides (Cys6-Cys47, Cys18-Cys61, Cys48-Cys52; N-Met IGF-I peak 1 peptide) demonstrated < 15% binding under similar conditions.Potency of the peptides was investigated in competitive IGF-I plasma binding protein and L6 myoblast radioreceptor assays. The peak 2 peptide proved equipotent to purified ovine plasma IGF-I in each system but by contrast the peak 1 peptide was 40-fold and 200-fold less potent in the binding protein and radioreceptor assays respectively.Biological potency was examined in a non-competitive assay based on incorporation of [3H]leucine into confluent cultures of L6 myoblasts. In this system the N-Met IGF-I peak 1 peptide proved 15-fold less potent than the peak 2 peptide with correct disulphide linkages.Refolding variants may prove useful in establishing structure/function relationships for IGF-I.

We also recommend Trading Suppliers and Manufacturers of insulin-like growth factor I, N-methionyl- (cas 106768-76-7). Pls Click Website Link as below: cas 106768-76-7 suppliers