Add time:08/07/2019         Source:sciencedirect.com

Autooxidation of hydroquinone (HQ) or 1,2,4-benzenetriol (BT), catalysed by hemin in the presence of dithiothreitol was studied in phosphate buffered saline. Inclusion of glutamate in the above reaction mixture resulted in the formation of thiobarbituric acid reactive products (TBAR) only in an aerobic atmosphere and was linear up to 2 h. Oxygen consumption was noticed during the reaction process. The formation of TBAR was linear with the increase in concentration of heme (1 – 4 μM), dithiothreitol (0.2 – 2 mM) or BT (0.17 – 0.85 mM). Linearity of TBAR formation from glutamate for up to 2 h was observed during the autooxidation of BT in the presence of heme. Besides glutamate, heme concentration dependent formation of TBAR from deoxyuridine or DNA was also observed. Almost complete inhibition of TBAR formation from glutamate, deoxyuridine or DNA was observed in the presence of catalase or superoxide dismutase (SOD). The presence of thiourea or mannitol in the reaction mixture caused substantial diminution of TBAR formation. Albumin or dimethyl sulfoxide also caused partial inhibition. Complete to partial inhibition observed in the presence of oxyradical scavengers in this study indicates that hemin catalysed autooxidation of BT results in the formation of reactive oxygen radicals.

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