Add time:08/05/2019 Source:sciencedirect.com
A novel rhodamine-based turn-on fluorescent probe with three thiourea receptors was designed, synthesized and fully characterized. In acetonitrile/4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid buffer (9/1, v/v, pH = 7.21), the probe showed high selectivity and sensitivity to Hg2+ with a 20-fold fluorescence enhancement at 583 nm. The increase in fluorescence intensity was linearly proportional to the concentration of Hg2+ in the range of 25–200 μM with a detection limit of 3.04 × 10−7 M. The probe could work in a nearly neutral pH span of 6.41–8.33 and exhibited excellent interference immunity. Real sample assay showed the probe had good practicability. The results from Job's plot, reversibility experiment, mass and infrared spectra analysis suggested a 1:3 probe/Hg2+ complex with an association constant of 9.88 × 1011 M−3 and a new interaction way between the probe and Hg2+.
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