Add time:08/06/2019         Source:sciencedirect.com

-Exogenously supplied 4-hydroxybenzoic acid (HBA) was converted quantitatively to 4-(β-d-glucopyranosyloxy) benzoic acid (HBA glucoside) during 30 hr of germination of the pollen of Pinus densiflora (pine pollen). [14C-carboxyl]HBA ([14C]-HBA) was also converted to [14C]-HBA glucoside by pine pollen. UDP glucose: 4-(β-d-glucopyranosyloxy) benzoic acid glucosyltransferase from pine pollen was assayed by measuring the rate of formation of [14C]-HBA glucoside from [14C]-HBA and UDPG. The enzyme was partially purified from the pine pollen for the first. The Mr of the enzyme was estimated to be ca 33 000 by Sephacryl S-300 gel filtration. The enzyme had a pH optimum at 7.5, and its activity was increased ca 50 and 60% by the addition of 3 mM Ca2+ and 0.1 mM EDTA, respectively. The Km values for UDP glucose and 4-hydroxybenzoic acid were 2.4 × 10−4 M and 2.9 × 10−3 M, respectively.

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