Add time:08/04/2019         Source:sciencedirect.com

The effect of 4-(1-naphthylvinyl)-pyridine (NVP), an inhibitor of choline acetyltransferase, was investigated on responses to several cholinomimetics and to nervous stimulation of the rat bladder. NVP antagonised responses to acetylcholine (ACh) as well as responses to nervous stimulation suggesting that it has an inhibitory action on the smooth muscle. Furthermore, the reduction in the responses to nervous stimulation was independent of the frequency of stimulation suggesting that inhibition of choline acetyltransferase was not a factor in the effect of NVP on nervous stimulation. Another finding was that NVP produced more marked inhibition of responses to ACh and methacholine than of those to carbachol (CCh) or tetramethylammonium. Responses of the bladder to Ba2+ and bradykinin were also antagonised by high concentrations of NVP. Negative inotropic responses of driven rat atria to ACh were also inhibited by NVP whereas responses to CCh were not affected. On rat ileum however, both agonists were affected to a similar extent. The results indicate that NVP is not useful as a tool to investigate the effect of selective inhibition of choline acetyltransferase in the isolated parasympathetically innervated tissues investigated because of its inhibitory effects on directly acting agonists. However, the more marked inhibition of responses to ACh than to CCh in some tissues suggests that it may be of value in detecting differences between the binding of these two agonists to the muscarinic receptor.

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