Add time:08/05/2019         Source:sciencedirect.com

Laccase production by Trametes pubescens grown on sunflower-seed shells (SS) under solid-state fermentation (SFF) conditions in temporary immersion bioreactors was studied. Three immersion cycles were considered: 1 min immersed and 9 min non-immersed, 1 min immersed and 30 min non-immersed and 1 min immersed and 60 min non-immersed. The latter led to the highest laccase activities (4000–6000 U l−1). Also, the in vitro and in vivo decolouration of the recalcitrant textile dye Remazol Brilliant Blue R (RBBR) was assessed. It was found that RBBR (133.33 mg l−1) was efficiently decolourised by T. pubencens grown on SS under SSF conditions in temporary immersion bioreactors in five successive batches. The percentage of RBBR decolouration was higher than 55% in 4 h and around 70% in 24 h in all the batches. However, it was found that RBBR decolouration by the crude culture filtrates was more advantageous. Thus, an RBBR decolouration percentage of nearly 80% in 2 h was obtained.

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