Add time:08/07/2019         Source:sciencedirect.com

Starches with comb-like profiles have been detected in some cereal endosperms with inhibiting expression of starch branching enzyme (SBE). Although amylose is considered to be an important factor in the formation of the comb-like profile, the details remain unclear. In this study, a transgenic rice line (GLXN-SBEI/IIb−) was derived from japonica waxy rice cultivar Guang-ling-xiang-nuo (GLXN) through antisense RNA inhibition of both SBEI and SBEIIb. The expression and activity of SBEI, SBEIIb and SBEIIa were declined. The GLXN-SBEI/IIb− endosperm contained large and small starch granules, and these starch granules had the comb-like profiles. The comb-like profiles of starches were detected in GLXN-SBEI/IIb− endosperm after 10 days after flowering with gradually increasing proportion of long branch-chains of amylopectin. The long branch-chains of amylopectin were responsible for forming the comb-like profiles at the outer region of starch granules. The gradually decreasing expression of SBEs influenced the synthesis of amylopectin during endosperm development, resulting in different structure between the inner and outer regions of starch granules from GLXN-SBEI/IIb− endosperm. The above results indicated that the long branch-chains of amylopectin, not amylose, led to the formation of comb-like profiles of starch granules in cereal crops with inhibiting expression of SBEs.

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