Add time:08/10/2019         Source:sciencedirect.com

An assessment was conducted to compare the precision and accuracy of Nessler, phenate, salicylate, and ammonia electrode procedures for total ammonia nitrogen (TAN) concentration determination in waters of aquaculture. Salicylate method was selected as a standard method for its high precision and accuracy. In replicate analyses of water samples for precision estimate, Nessler method usually gave higher mean concentration of TAN than other methods and it is not recommended for use for its high coefficient of variance. Results from Nessler's with Rochelle salt method were more accurate than Nessler without Rochelle salt method. Electrodes for sensing NH3 and NH4+ usually gave higher mean concentration of TAN than salicylate method. NH4+ electrode, although with high accuracy, was strongly affected by sodium and potassium and not recommended to use especially in waters of high salinity. The finding also suggested that the salicylate kit by using YSI photometer also was a preferable alternative to salicylate method while Nessler kit was not. TAN concentrations measured in 27 water samples by all methods were highly correlated (R2 = 0.919 to 0.996) with salicylate method. All of the slopes except phenate I and II method were different from 1.0 (P < 0.05); all of the intercepts except phenate I and salicylate kit were different from 0.0 (P < 0.05).

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