Add time:08/09/2019         Source:sciencedirect.com

The first-order UV-derivative spectrophotometry, applying zero-crossing method was developed for the determination of omeprazole (OM), omeprazole sulphone (OMS), pantoprazole sodium salt (PANa), and N-methylpantoprazole (NPA) in methanol–ammonia 4.0% v/v, where the sufficient spectra resolutions of drug and corresponding impurity were obtained, using the amplitudes 1D304, 1D307, 1D291.5 and 1D296.5, respectively. Method showed good linearity in the ranges (μg ml−1): 1.61–17.2 for OM; 2.15–21.50 for OMS; 2.13–21.30 for PANa and 2.0–20.0 for NPA, accuracy and precision (repeatability and reproducibility). The experimentally determined values of LOD (μg ml−1) were 1.126; 0.76; 0.691 and 0.716 for OM, OMS, PANa and NPA, respectively. The obtained values of 2.91% w/w for OMS and 3.58% w/w for NPA in the presence of their parent drug, by applying the method of standard additions, point out the usage of the proposed method in stability studies. Zero-crossing method in the first-order derivative spectrophotometry showed the impurity–drug intermolecular interactions, due to the possible intermolecular hydrogen bonds, confirmed by divergences of experimentally obtained amplitudes for impurities OMS and NPA in comparison to expected values according to regression equations of calibration graphs.

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