Add time:08/09/2019 Source:sciencedirect.com
To efficiently produce 1,3-Adamantanediol (cas 17071-62-4) (1,3-ad(OH)2) from 1-adamantanol (1-adOH), our stocks of culture strains and soil microorganisms were surveyed for hydroxylation activity towards 1-adOH. Among them, the soil actinomycete SA8 showing the highest hydroxylation activity was identified as Streptomyces sp. based on 16S ribosomal DNA sequence analysis. The reaction products were purified by silica gel column chromatography, and from NMR and MS analyses, they were identified as 1,3-ad(OH)2 and 1,4-ad(OH)2. Streptomyces sp. SA8 produced 5.9 g l− 1 1,3-ad(OH)2from 6.2 g l− 1 1-adOH in culture broth after 120 h at 25 °C. Using resting cells, 2.3 g l− 1 1,3-ad(OH)2 was produced after 96 h of incubation at a 69% conversion rate. In both cases, 1,4-ad(OH)2 was formed as a byproduct at a rate of about 15%. Strain SA8 also hydroxylated 2-adamantanol and 2-methyl-2-adamantanol.
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