Add time:08/10/2019         Source:sciencedirect.com

Antibodies directed against human milk sialyloligosaccharides [D. F. Smith and V. Ginsburg (1980) J. Biol. Chem.255, 55–59] are used to identify human meconium gangliosides by radioimmuneoverlay-thin-layer chromatography or by direct binding on nitrocellulose filters of sialyl[3H]oligosaccharide alditols obtained from gangliosides after ozonolysis and alkali-fragmentation. Thin-layer chromatograms of meconium monosialylgangliosides immunostained with rabbit antisera specific for LS-tetrasaccharide c (NeuAcα2-6Galβ1-4GlcNAcβ1-3Galβ1-4Glc) or LS-tetrasaccharide b (Galβ1-3[NeuAcα2-6]GlcNAcβ1-3Galβ1-4Glc) reveal their corresponding gangliosides, 6′-LM1 and a previously undescribed ceramide derivative of LS-tetrasaccharide b, respectively. The sialyl[3H]oligosaccharides derived from the monosialylganglioside fraction of meconium are separated by paper chromatography and assayed for binding to specific anti-sialyloligosaccharide sera. Antisera specific for LS-tetrasaccharide c and 3′-sialyllactose (NeuAcα2-3Galβ1-4Glc) identify their corresponding 3H-labeled haptens released from the major meconium gangliosides 6′-LM1 and GM3, respectively. Binding of a ganglioside-derived sialyl[3H]oligosaccharide by anti-LS-tetrasaccharide b serum is consistent with the presence in meconium of a monosialylganglioside with the following proposed structure:

We also recommend Trading Suppliers and Manufacturers of LS tetrasaccharide d (cas 100789-83-1). Pls Click Website Link as below: cas 100789-83-1 suppliers