Add time:08/10/2019         Source:sciencedirect.com

Branched cyclomalto-oligosaccharides are formed when pullulanase from Bacillus acidopullulyticus is incubated with α-maltosyl fluoride (α-G2F) as substrate in the presence of cyclomalto-oligosaccharides as acceptor. It was shown by enzymic and chemical methods that the structures of these branched cyclomalto-oligosaccharides are 6-O-α-maltosylcyclomaltohexaose (G2-cG6), 6,6′-di-O-α-maltosylcyclomaltohexaose [(G2)2-cG6], 6-O-α-maltosylcyclomaltoheptaose (G2-cG7), 6,6′-di-O-α-maltosylcyclomaltoheptaose [(G2)2-cG7], and 6,6′,6″-tri-O-α-maltosylcyclomaltoheptaose [(G2)3-cG7]. The optimal conditions for formation of G2-cG6 were studied. About 12mm G2-cG6 was produced when pullulanase (2.8 U/mL) was incubated with 40mm α-G2F as substrate and 90mm cyclomaltohexaose (cG6) as acceptor for 1 h at 60°. The yield of G2-cG6, the main transfer-product, was twenty times that by the condensation of cG6 with α-maltose in the presence of pullulanase.

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