Add time:08/11/2019 Source:sciencedirect.com
The chemiluminescence reaction between an aryl oxalate, hydrogen peroxide and a fluorescent compound is well known for use in h.p.l.c. post-column reactors. Here, several aryl oxalates are evaluated for this purpose in terms of intensity, rate of chemiluminescence decay, solubility in different solvents, and stability in the presence of hydrogen peroxide. Five oxalates are selected for different pH ranges of column eluates: bis(pentafluorophenyl) oxalate for pH < 2, bis(2,4-dinitrophenyl) oxalate for pH 2–4, bis(2-nitrophenyl) oxalate for pH 4–6, bis(2,4,6-trichlorophenyl) oxalate for pH 6–8, and bis(2,4,5-trichlorophenyl-6-pentyloxycarbonyl) oxalate for pH > 8.
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