Add time:08/11/2019         Source:sciencedirect.com

Summary3-Hydroxy-5,8,11,14-eicosatetraenoic acid (3-HETE) is a biologically active compound which has a signal transducing role in human neutrophils. When 12.5 mg/l arachidonic acid (AA) was fed to sexual reproductive cells of Dipodascopsis uninucleata and D. tóthii the former transformed approximately 4% of AA to 3-HETE, while the latter produced only trace amounts of 3-HETE. The 3-HETE was only associated with cellular ethanol extracts of both species. D. uninucleata utilized 69% of the AA after 6h while D. tóthii utilized 33%. The residual or unused AA was mainly present in the cells of D. tóthii (96%) and D. uninucleata (98%) and only small amounts (4% and 2%) in their respective supernatants. Extraction with 80% aqueous ethanol succeeded in removing respectively 92% and 45% of AA present in the cells of D. tóthii and D. uninucleata. The poor AA extraction from cells of D. uninucleata contributed to the presence of AA in the neutral lipid fraction which is insoluble in ethanol. If efficient AA extraction can be implemented, approximately 67% and 31% unused AA can be recovered from D. tóthii and D. uninucleata respectively which can be recycled for biotransformation purposes. Although no traces of other 3-hydroxy fatty acids were detected, enzyme extracts from D. uninucleata metabolised exogenous 18:2 in a manner consistent with the presence of a prostaglandin endoperoxide synthase similar to that in fetal calf blood vessels.

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