Add time:08/11/2019         Source:sciencedirect.com

An electron-mediated glucose biosensor was constructed from spectroscopic-grade graphite electrodes in three steps. A non-conducting electropolymerized film serves to screen out electroactive interferents and prevents electrode fouling. The electron mediator 1,1′-dimethylferrocene is adsorbed on, and retained by the polymer film-modified electrode. Glucose oxidase is then crosslinked with glutaraldehyde on the modified electrode. This yields a biosensor with linear glucose response (2–76 mM) at +150 mV vs. SCE, and little or no interference from oxygen, with a lifetime of up to four months.

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