Add time:08/15/2019         Source:sciencedirect.com

Laccase from Trametes versicolor was immobilized on Amberlite IR-120 H beads. Maximum immobilization obtained was 78.7% at pH = 4.5 and temperature T = 45 °C. Kinetic parameters, Km and Vmax values, were determined respectively as 0.051 mM and 2.77 × 10−2 mM/s for free and 4.70 mM and 5.27 × 10−3 mM/s for immobilized laccase. The Amberlite–laccase system showed a 30% residual activity after 7 cycles. On the other hand, the loss of activity for free laccase after 7 days of storage at 4 °C was 18.5% in comparison to Amberlite–laccase system with a loss of 1.4%, during the same period. Improved operational, thermal and storage stabilities of the immobilized laccase were obtained compared to the free counterpart. Therefore, the use of low-cost matrices, like Amberlite for enzyme immobilization represents a promising product for enzymatic industrial applications.

We also recommend Trading Suppliers and Manufacturers of AMBERLITE MB-150 (cas 100915-96-6). Pls Click Website Link as below: cas 100915-96-6 suppliers