Add time:08/16/2019         Source:sciencedirect.com

Response surface methodology was used to model and optimise the production of Ethyl butyrate (cas 105-54-4), catalysed by Rhizopus oryzae lipase immobilised in a hydrophilic polyurethane foam. Experiments were carried out following a central composite rotatable design, as a function of reaction temperature (T: 22–38 °C) initial butyric acid concentration (A: 0.031–0.619 M) and initial molar ratio ethanol/acid (MR; 0.257–2.443). After 48 h reaction time, the production of ethyl butyrate could be fitted to a surface described by a second-order polynomial model. A maximum ethyl butyrate concentration of 0.106 M, corresponding to 47% conversion into ester and a productivity of 2.21 μmole/mL h, is expected at initial reaction conditions of T, A and MR of 33 °C, 0.225 M and 1.637, respectively. This maximum was experimentally confirmed.

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