Add time:08/17/2019         Source:sciencedirect.com

Pseudomonas putida strain NCIB9866 (RA4000) carries an 85-kb plasmid, pRA4000, can utilize p-cresol and 2,4-Xylenol (cas 105-67-9), and is resistant to inorganic mercuric ions. The loss of this phenotype from a pRA4000-cured derivative, combined with DNA analysis and conjugation studies, showed that pRA4000 encodes the degradation of p-cresol and resistance to inorganic mercuric ions. Studies using phenotypic variants of RA4000 altered in their ability to degrade 2,4-xylenol and p-cresol indicated that structural genes for 2,4-xylenol utilization are located on the chromosome and that their expression requires a positive regulator encoded on pRA4000.

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